Title of article :
A novel HPLC procedure for detection and quantification of aminoacetone, a precursor of methylglyoxal, in biological samples
Author/Authors :
Kazachkov، نويسنده , , Michael K. Yu، نويسنده , , Peter H.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2005
Pages :
7
From page :
116
To page :
122
Abstract :
Increase in methylglyoxal is thought to be involved in different pathological conditions. Deamination of aminoacetone by semicarbazide-sensitive amine oxidase (SSAO) leads to production of methylglyoxal. We have synthesized aminoacetone and developed a novel HPLC procedure for its quantitative determination. The urinary excretion of aminoacetone is approximately 20–30 μg/mouse/day, and the concentration is about 0.5 μg/g in mouse liver and small intestine. SSAO inhibitor increases aminoacetone levels in both tissues and urines. Results confirm that aminoacetone is an endogenous substrate for SSAO. However, data also indicate that deamination is not the only catabolic pathway for aminoacetone.
Keywords :
Aminoacetone , Methylglyoxal , SSAO , HPLC
Journal title :
Journal of Chromatography B
Serial Year :
2005
Journal title :
Journal of Chromatography B
Record number :
1462147
Link To Document :
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