Development of a solid phase microextraction–gas chromatography method to determine N-hydroxymethyl-N-methylformamide and N-methylformamide in urine

A headspace solid phase microextraction (SPME) method has been developed to determine metabolites of dimethylformamide, N-hydroxymethyl-N-methylformamide, and N-methylformamide (NMF) as NMF in urine by gas chromatography with nitrogen–phosphorus detector (GC-NPD). An SPME holder with a 65-μm PDMS/DVB fiber coating was used. Optimal desorption conditions were 250 °C for 1 min, adsorption at 80 °C for 15 min, and 3.00 mL of sample in the headspace vial. The method presented good resolution, repeatability, recovery, detection limit, ruggedness and response linearity.