Determination of scutellarin in mouse plasma and different tissues by high-performance liquid chromatography

A simple HPLC–UV method was developed for the determination of scutellarin in plasma and different tissues of mice (heart, liver, spleen, lungs and kidneys). The separation was achieved by HPLC on a Hypersil C18 column with a mobile phase composed of methanol–water–glacial acetic acid (40:60:1). UV detection was used at 335 nm. The calibration curves were linear in all matrices (r2 > 0.997) in the concentration range of 0.1–10 μg/ml for plasma and 0.1–20 μg/g for tissue homogenates, respectively. The method described is suitable for studies on the distribution of scutellarin in different tissues of mice.