Application of l-Amino acid in determination of Huperzine A by high performance liquid chromatography

A selective HPLC method for determination of Huperzine A in Huperzia serrata Extract has been developed and validated. Huperzine A was dissolved in 0.01 mol/L HCl, chromatographed on an Agilent Zorbax SB-C18 (150 mm × 4.6 mm i.d., 5 μm) column, with a mobile phase consisting of methanol-1 mM l-Lysine water solution (50:50, v/v), and detected at 310 nm. The UV peak areas were used for quantitation of Huperzine A content. The correlation coefficient (R2) of the calibration was 0.9999 over the range of 1–25 μg/ml and intra- and interday precision over this range were not more than 2%. The method was successfully applied to characterize and determine the Huperzine A in Huperzia serrata Extract.