Title of article
High-performance liquid chromatographic method for the determination of histamine and 1-methylhistamine in biological buffers
Author/Authors
von Vietinghoff، نويسنده , , Vivica and Gنbel، نويسنده , , Gotthold and Aschenbach، نويسنده , , Jِrg R.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2006
Pages
5
From page
335
To page
339
Abstract
A method for the determination of histamine and its catabolite 1-methylhistamine (1-MH) was developed, using HPLC with fluorescence detection. Derivatization of both compounds occurred on-column with o-phthaldialdehyde dissolved in an alkaline borate buffer, followed by separation on a reversed phase C18 column. Histamine and 1-MH could be detected with comparable sensitivity (limit of quantification, 50 nM). The method was proven suitable to investigate catabolism of histamine by epithelia of pig colon. The method should be useful in research on histamine metabolism.
Keywords
1-Methylhistamine , Histamine release , Intestine , Histamine metabolism , High-performance liquid chromatography , histamine , Histamine absorption , Ussing chamber
Journal title
Journal of Chromatography B
Serial Year
2006
Journal title
Journal of Chromatography B
Record number
1463678
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