Author/Authors :
Zhao، نويسنده , , Ming and Rudek، نويسنده , , Michelle A. and He، نويسنده , , Ping and Hafner، نويسنده , , Frank-Thorsten and Radtke، نويسنده , , Martin C Wright، نويسنده , , John J. and Smith، نويسنده , , B. Douglas and Messersmith، نويسنده , , Wells A. and Hidalgo، نويسنده , , Manuel and Baker، نويسنده , , Sharyn D.، نويسنده ,
Abstract :
A rapid, sensitive and specific method was developed and validated using LC/MS/MS for determination of sorafenib in human plasma. Sample preparation involved a single protein precipitation step by the addition of 0.1 mL of plasma with 0.5 mL acetonitrile. Analysis of the compounds of interest including the internal standard ([2H3 15N] sorafenib) was achieved on a Waters X-Terra™ C18 (150 mm × 2.1 mm i.d., 3.5 μm) analytical column using a mobile phase consisting of acetonitrile/10 mM ammonium acetate (65:35, v/v) containing 0.1% formic acid and isocratic flow at 0.2 mL/min for 6 min. The analytes were monitored by tandem mass spectrometry with electrospray positive ionization. Linear calibration curves were generated over the range of 7.3–7260 ng/mL for the human plasma samples with values for the coefficient of determination of >0.96. The values for both within day and between day precision and accuracy were well within the generally accepted criteria for analytical methods (<15%).
