Analytical method for the quantitative determination of cyanuric acid as the degradation product of sodium dichloroisocyanurate in urine by liquid chromatography mass spectrometry

A simple and selective analytical method for the quantitative determination of cyanuric acid, the degradation product of sodium dichloroisocyanurate (NaDCC), in human urine is reported herein. The sample preparation involved the use of diatomaceous earth extraction columns. Quantification was achieved by liquid chromatography mass spectrometry using negative ion electrospray with a cyano (CN) column. Between day relative standard deviation less than 10% (n = 6) was obtained at the 5 mg L−1 level. The assay was linear over the investigated range 0–20 mg L−1 and the limit of detection (LOD) was confirmed to be 0.1 mg L−1. The method was applied to monitoring levels of cyanuric acid in healthcare workers using disinfectants products containing NaDCC.