Author/Authors :
Wen، نويسنده , , Aidong and Wang، نويسنده , , Zhipeng and Hang، نويسنده , , Taijun and Jia، نويسنده , , Yanyan and Zhang، نويسنده , , Tingting and Wu، نويسنده , , Yin and Gao، نويسنده , , Xuan and Yang، نويسنده , , Zhifu، نويسنده ,
Abstract :
A simple, rapid, sensitive and reliable high performance liquid chromatography (HPLC) method for the determination of the anti-ulcer drug sofalcone in human plasma was developed. Plasma was extracted with ethyl acetate under acidic conditions and sofalcone was determined by HPLC using a C18 column and (methanol–0.1% formic acid aqueous 80:20) mobile phase. The linear calibration curves of sofalcone in human plasma were obtained over the concentration range of 0.01–5.0 μg/ml. The lower limit of quantitation (LLOQ) was 10 ng/ml in human plasma. The precision measured for plasma was within 15%. Extraction recovery was over 85% in blood. The method was successfully applied to the identification and quantification of sofalcone in pharmacokinetic studies.
