Title of article :
Fast and direct quantification of adrenal steroids by tandem mass spectrometry in serum and dried blood spots
Author/Authors :
Janzen، نويسنده , , Nils and Sander، نويسنده , , Stefanie and Terhardt، نويسنده , , Eric Dupont
Michael Nicolaidis
Peter Rohr
، نويسنده , , Michael and Sander، نويسنده , , Johannes، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2008
Abstract :
We present a fast and reproducible method for steroid analysis (corticosterone, deoxycorticosterone, progesterone, 17α-hydroxyprogesterone, 11-deoxycortisol, 21-deoxycortisol, androstenedione, testosterone, dihydrotestosterone and cortisol) in small volumes of serum and in dried blood spot samples by LC-MS/MS. No derivatisation was needed. LC separation was achieved by using an Atlantis® C18 column and water–methanol–formic acid gradient as a mobile phase and a flow rate of 250 μL/min over a run time of 6 min. Steroids were measured in MRM mode with electrospray interface (positive ion mode). Validation showed excellent precision, sensitivity, recovery and linearity with coefficients of determination r2 > 0.992.
Keywords :
Adrenal cortex disorders , Tandem mass spectrometry , Steroid profile
Journal title :
Journal of Chromatography B
Journal title :
Journal of Chromatography B