Title of article :
Simultaneous quantitation of dexamethasone palmitate and dexamethasone in human plasma by liquid chromatography/tandem mass spectrometry
Author/Authors :
Yang، نويسنده , , Yan and Li، نويسنده , , PIAO Song-hao and GAO Chao، نويسنده , , Kan and Liu، نويسنده , , Mingyuan and Sun، نويسنده , , Yantong and Yan، نويسنده , , Tingting and Fawcett، نويسنده , , J. Paul and Cui، نويسنده , , Yimin and Gu، نويسنده , , Jingkai، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2008
Pages :
6
From page :
119
To page :
124
Abstract :
A rapid and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for simultaneous quantitation of dexamethasone palmitate and dexamethasone in human plasma was developed. After sample preparation by protein precipitation and liquid–liquid extraction, the analytes and internal standard (IS) were separated on a Venusil XBP-C8 column using gradient elution. Multiple reaction monitoring of dexamethasone palmitate, dexamethasone and IS used the precursor to product ion transitions at m/z 631.8 → 373.1, m/z 393.2 → 147.1 and m/z 264.2 → 58.1, respectively. The method was linear over the ranges 1.5–1000 ng/mL for dexamethasone palmitate and 2.5–250 ng/mL for dexamethasone with intra- and inter-day precisions of <10% and accuracies of 100 ± 7%. The assay was applied to a clinical pharmacokinetic study involving the injection of dexamethasone palmitate to healthy volunteers.
Keywords :
dexamethasone , Dexamethasone palmitate , LC/MS/MS , Pharmacokinetics , human
Journal title :
Journal of Chromatography B
Serial Year :
2008
Journal title :
Journal of Chromatography B
Record number :
1465584
Link To Document :
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