Title of article :
Enantiospecific gas chromatographic–mass spectrometric analysis of urinary methylphenidate: Implications for phenotyping
Author/Authors :
LeVasseur، نويسنده , , Natalie L. and Zhu، نويسنده , , Hao-Jie and Markowitz، نويسنده , , John S. and DeVane، نويسنده , , C. Lindsay and Patrick، نويسنده , , Kennerly S. and Donovan، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2008
Pages :
10
From page :
140
To page :
149
Abstract :
A chiral derivatization gas chromatographic–mass spectrometric (GC–MS) method for urine methylphenidate (MPH) analysis was developed and validated to investigate preliminary findings regarding a novel MPH poor metabolizer (PM). Detection was by electron impact (EI) ionization-selected ion monitoring of the N-trifluoroacetylprolylpiperidinium fragments from MPH and the piperidine-deuterated MPH internal standard. The PM eliminated ∼70 times more l-MPH in urine (9% of the dose over 0–10 h), and ∼5 times more of the d-isomer (10% of the dose), than the mean values determined from 10 normal metabolizers of MPH. Only minor amounts of the metabolite p-hydroxy-MPH were found in the urine of both the PM and normal metabolizers, while the concentration of MPH lactam was not high enough to be detectable. The described method indirectly gauges the functional carboxylesterase-1 status of patients receiving MPH based on the evaluation of relative urine concentrations of d-MPH:l-MPH. Clinical implications concerning rational drug selection for an identified or suspected MPH PM are discussed.
Keywords :
methylphenidate , esterase , Enantiomers , urine , pharmacogenetics
Journal title :
Journal of Chromatography B
Serial Year :
2008
Journal title :
Journal of Chromatography B
Record number :
1465591
Link To Document :
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