Title of article
Sample preparation method for plasma membrane proteome analysis
Author/Authors
Park، نويسنده , , Seung-Ah and Kim، نويسنده , , Mi-Ryung and Kim، نويسنده , , Pan-Kyeom and Cho، نويسنده , , Hyung-Don and Han، نويسنده , , Gi-Yeon and Kim، نويسنده , , Chan-Wha Kim، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2008
Pages
4
From page
177
To page
180
Abstract
The preparation of plasma membrane (PM) proteome samples is seriously difficult and time-consuming, owing to their profound hydrophobicity and low abundance. We have developed an efficient PM sample preparation method using Ultracentrifugation with Percoll and an aqueous two-phase extraction. The developed method was rapid (3 h) and provided high purities (26-fold of cell lysate) with a high yield (2.6% of whole cell lysate proteins). This method is especially useful for PM proteome studies using 2D gel electrophoresis.
Keywords
hMSCs , Percoll , plasma membrane , Two-phase extraction , Two-dimensional electrophoresis , ultracentrifugation
Journal title
Journal of Chromatography B
Serial Year
2008
Journal title
Journal of Chromatography B
Record number
1466315
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