Title of article :
Validated method for determination of mazindol in human plasma by liquid chromatography/tandem mass spectrometry
Author/Authors :
Kim، نويسنده , , Sung Su and Lee، نويسنده , , Heon-Woo and Lee، نويسنده , , Kyung-Tae، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2009
Pages :
6
From page :
1011
To page :
1016
Abstract :
A simple liquid chromatography/tandem mass spectrometry (LC/MS/MS) method with electrospray ionization in positive ion multiple reaction monitoring mode was developed for the quantification of mazindol (an anorectic agent) in human plasma. Fluoxetine was adopted as an internal standard (IS), and sample preparation involved one-step liquid/liquid extraction using ethyl acetate. The transition monitored were m/z 285 > 44 for mazindol and m/z 310 > 44 for IS. Chromatographic separation was achieved on a Capcell Pak MGII C18 column using an isocratic mobile phase, consisting of acetonitrile–20 mM ammonium formate in water (50:50, v/v, adjusted to pH 3.5 with formic acid) at a flow-rate of 0.2 mL/min. The retention times of mazindol and fluoxetine were 1.03 min and 1.45 min, respectively. The lower limit of quantitation (LLOQ) was 0.1 ng/mL using 200 μL of plasma, and no interferences were detected in chromatograms. The bench top stability of mazindol was evaluated in buffered and non-buffered plasma. The selectivity, linearity, precision, accuracy, recovery, and stability of the devised method were fully validated and absolute and relative matrix effects were evaluated. The described method provides a fast and sensitive analytical tool for determining mazindol levels in plasma, and was successfully applied to a pharmacokinetic study in 24 healthy human subjects after oral administration of 2 mg tablet formulation of mazindol under fasting conditions.
Keywords :
Human plasma , Pharmacokinetic study , LC/MS/MS , Mazindol
Journal title :
Journal of Chromatography B
Serial Year :
2009
Journal title :
Journal of Chromatography B
Record number :
1467050
Link To Document :
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