Title of article :
Dynamic lipidomics with stable isotope labelling
Author/Authors :
Postle، نويسنده , , Anthony D. and Hunt، نويسنده , , Alan N.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2009
Pages :
6
From page :
2716
To page :
2721
Abstract :
Incorporation of stable isotope labelled precursors enables estimation of the kinetics of lipid synthesis and turnover (dynamic lipidomics) in the clinical as well the experimental setting. Recent advances in tandem mass spectrometry extend the analytical possibilities from measurements of isotope enrichments to determinations of intact substrates. Incorporations of deuteriated choline, ethanolamine and inositol can be determined by precursor and neutral loss scans of phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol, respectively. This experimental approach provides information on the kinetics of individual phospholipid molecular species and has considerable potential to probe diseases of lipid metabolism in vivo.
Keywords :
Dynamic lipidomics , stable isotopes , lipid metabolism , Phospholipid synthesis
Journal title :
Journal of Chromatography B
Serial Year :
2009
Journal title :
Journal of Chromatography B
Record number :
1467546
Link To Document :
بازگشت