Title of article
Concurrent extraction of proteins and RNA from cell-laden hydrogel scaffold free of polysaccharide interference
Author/Authors
Wang، نويسنده , , Chunming and Li، نويسنده , , Xin and Yao، نويسنده , , Yongchang and Wang، نويسنده , , Dong-An، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2009
Pages
5
From page
3762
To page
3766
Abstract
Concurrent extractions of proteins and RNA from cell-laden scaffolds would be of great help to biomaterialists and tissue engineers. Here we describe a procedure to extract proteins from the discard solution generated during the RNA isolation from polysaccharide-rich hydrogel scaffolds. This approach allows to obtain proteins and RNA from same sample while eliminating the polysaccharide interference.
Keywords
polysaccharide , mass spectrometry , protein extraction , RNA , Biomaterials , Tissue engineering
Journal title
Journal of Chromatography B
Serial Year
2009
Journal title
Journal of Chromatography B
Record number
1467848
Link To Document