Simultaneous analysis of fluoroquinolones and xanthine derivatives in serum by molecularly imprinted matrix solid-phase dispersion coupled with liquid chromatography

A new molecularly imprinted polymer was synthesized using ofloxacin and theophylline as template and methacryclic acid as function monomer and it was employed as a special dispersant of matrix solid-phase dispersion for selective extraction of fluoroquinolones (ofloxacin, ciprofloxacin and enrofloxacin) and xanthine (caffeine and theophylline) from human serum samples. To eliminate the influences of template leaking on quantitative analysis, acetonitrile–trifluoracetic acid (99:1, v/v) was used as the template removing solution. By using water and acetonitrile–trifluoracetic acid (99.5:0.5, v/v) as the washing and elution solvent, respectively, satisfactory recoveries and clean enough chromatogram could obtained. Good linearity of all the analytes was observed in a range of 0.35–150 μg g−1 with the correlation coefficient (r2) ≥ 0.9991. The recoveries of spiked human serum samples were in a range of 89.5–104.0% for fluoroquinolones and xanthine derivatives with RSD less than of 5.0%.