Author/Authors :
Zhang، نويسنده , , Youxi and Sun، نويسنده , , Jin and Gao، نويسنده , , Yikun and Kong، نويسنده , , Ying and Xu، نويسنده , , Youjun and Jia، نويسنده , , Weiru and Liao، نويسنده , , Chuanrong and Zhang، نويسنده , , Peng and Lian، نويسنده , , He and Han، نويسنده , , Xiaopeng and Li، نويسنده , , Dongpo and Geng، نويسنده , , Yajie and He، نويسنده , , Zhonggui Chen، نويسنده ,
Abstract :
A simple and sensitive HPLC–MS/MS method was developed and validated for the simultaneous determination of decitabine and valdecitabine in rat plasma. The analytes were separated on a C18 column (150 mm × 4.6 mm, 3.5 μm) and a triple-quadrupole mass spectrometer equipped with an electrospray ionization (ESI) source was applied for detection. A clean solid-phase extraction procedure with cation exchange cartridge was employed to extract the analytes from rat plasma with high recovery of decitabine (>82%). The calibration curves were linear over a concentration range of 10–10,000 ng/mL for decitabine and 5–500 ng/mL for valdecitabine. The lower limit of quantitation (LLOQ) of decitabine and valdecitabine was 10 and 5 ng/mL, respectively. The intra-day and inter-day precisions were less than 15% and the relative error (RE) was all within ±15%. The validated method was successfully applied to a pharmacokinetics study in rats after either decitabine or valdecitabine orally administrated to the Sprague-Dawley rats.
Keywords :
HPLC–MS/MS , Simple solid-phase extraction , Pharmacokinetics , Rat plasma , Valdecitabine , Decitabine
