Title of article
Analysis of neutral lipids from microalgae by HPLC-ELSD and APCI-MS/MS
Author/Authors
Donot، نويسنده , , F. and Cazals، نويسنده , , G. and Gunata، نويسنده , , Z. and Egron، نويسنده , , D. and Malinge، نويسنده , , J. R. Strub، نويسنده , , C. and Fontana، نويسنده , , A. and Schorr-Galindo، نويسنده , , S.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2013
Pages
9
From page
98
To page
106
Abstract
A method was developed to analyze neutral lipids through the use of three triglycerides, four free fatty acids, six di- and four mono-glycerides standards by high performance liquid chromatography (HPLC) normal phase coupled with either with evaporative light scattering detector (ELSD) or with mass spectrometry (MS) operating in atmospheric pressure chemical ionization (APCI) mode. The method was applied to the determination of the neutral lipid fraction from a Botryococcus braunii race A (B. braunii) culture. This method led us to identify neutral lipids synthesized by B. braunii in a single analysis within 45 min through HPLC–APCI-MS/MS technique.
Keywords
Microalgae , HPLC , APCI-MS/MS , SPE , ELSD , Neutral lipids
Journal title
Journal of Chromatography B
Serial Year
2013
Journal title
Journal of Chromatography B
Record number
1471699
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