Title of article :
Application of ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry to determine the metabolites of orientin produced by human intestinal bacteria
Author/Authors :
Xu، نويسنده , , Jun-Min Qian، نويسنده , , Dawei and Jiang، نويسنده , , Shu and Guo، نويسنده , , Jianming and Shang، نويسنده , , Er-xin and Duan، نويسنده , , Jin-ao and Yang، نويسنده , , Jing، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2014
Pages :
5
From page :
123
To page :
127
Abstract :
An ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) with MetaboLynx™ software combined with mass defect filtering (MDF) method were provided for orientin metabolism study. The chromatographic separation was performed on a 1.7 μm particle size Syncronis C18 column using gradient elution system. The components were identified and confirmed according to the mass spectrometric fragmentation mechanisms, MS/MS fragment ions and relevant literature by means of electrospray ionization mass spectrometry in negative ion mode. With this method, a total of three metabolites were identified based on retention time and MS/MS data. The results illustrated that deglycosylation, dehydroxylation and acetylation were the major metabolic pathways of orientin in vitro by human intestinal bacteria. Additionally, colonic bacteria were screened for bacteria involved in the conversion of orientin. A gram-negative anaerobic bacterium, strain 45, was newly isolated from healthy human feces. This strain, which was able to cleave the C-glycoside of orientin to produce luteolin and generate some other metabolites, had the similarity of 95.44% with Enterococcus casseliflavus and was named Enterococcus sp. 45 based on 16S rRNA sequence analysis. In this paper, the metabolic routes, metabolites of oreintin produced by the intestinal bacteria and the Enterococcus sp. 45 were investigated for the first time.
Keywords :
Enterococcus sp. 45 , Orientin , 16S rRNA , UPLC-Q-TOF/MS , metabolites
Journal title :
Journal of Chromatography B
Serial Year :
2014
Journal title :
Journal of Chromatography B
Record number :
1471767
Link To Document :
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