Title of article :
Validation of an LC–MS/MS method for the quantification of mycophenolic acid in human kidney transplant biopsies
Author/Authors :
Md Dom، نويسنده , , Zaipul I. and Noll، نويسنده , , Benjamin D. and Coller، نويسنده , , Janet K. and Somogyi، نويسنده , , Andrew A. and Russ، نويسنده , , Graeme R. and Hesselink، نويسنده , , Dennis A. and van Gelder، نويسنده , , Teun and Sallustio، نويسنده , , Benedetta C. Sallustio، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2014
Pages :
7
From page :
171
To page :
177
Abstract :
Mycophenolic acid (MPA) has a low therapeutic index and large inter-individual pharmacokinetic variability necessitating therapeutic drug monitoring to individualise dosing after transplantation. There is an ongoing discrepancy as to whether plasma MPA concentrations sufficiently predict kidney rejection or toxicity and whether immunosuppressant concentrations within the graft tissue may better predict transplant outcomes. The aim of the study was to develop an LC–MS/MS method for the quantification of MPA concentrations in human kidney biopsies taken as part of routine clinical procedures. A total of 4 surplus human kidney biopsies obtained from 4 different kidney transplant recipients were available to use for this study. MPA was also quantified in 2 kidney samples from rats administered MPA to assess tissue extraction reproducibility. Human kidney biopsies and rat kidneys were homogenised mechanically and underwent liquid–liquid extraction before analysis by LC–MS/MS. MPA-free human kidney tissue was used in calibrators and quality control samples. Analyte detection was achieved from multiple reaction monitoring of the ammonium adducts of both MPA (m/z 321.1 → 207.3) and N-phthaloyl-l-phenylalanine (PPA, internal standard, m/z 296.2 → 250.2) using positive electrospray ionisation. The method was linear (calibration curves R2 > 0.99, n = 10), precise, and accurate with coefficients of variation and bias less than 15%. Extraction efficiencies for MPA and PPA were approximately 97% and 86%, respectively, and matrix effects were minimal. In 4 kidney transplant recipients, tissue MPA concentrations ranged from 1.3 to 7.7 ng/mg of tissue, however, the correlation between blood (C0) and tissue MPA concentrations could not be established. The method was successfully applied to the quantification of MPA in human kidney biopsies without the need to alter current clinical protocols.
Keywords :
Mycophenolic acid , Transplantation , LC–MS/MS , Tissue MPA concentrations
Journal title :
Journal of Chromatography B
Serial Year :
2014
Journal title :
Journal of Chromatography B
Record number :
1471846
Link To Document :
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