Author/Authors :
Liu، نويسنده , , Yuanyan and Liu، نويسنده , , Zhenli and Lu، نويسنده , , Cheng and Li، نويسنده , , Jianbei and Ning، نويسنده , , Zhangchi and Song، نويسنده , , Zhiqian and Wang، نويسنده , , Chun and Du، نويسنده , , Zhiyong and Lu، نويسنده , , Xinmiao and Zhao، نويسنده , , Siyu and Lu، نويسنده , , Aiping، نويسنده ,
Abstract :
Frankincense resins are extensively used as natural remedies in regions ranging from North Africa to China. Triterpenoid metabolites from frankincense exhibit notable anti-inflammatory and anti-tumor properties. In the present paper, without the use of an isolation process, the fragmentation rules and NMR spectral characteristics of triterpenoid metabolites in frankincense are summarized through a coupling method using high performance liquid chromatography-diode array detection/electrospray ionization tandem mass spectrometry (HPLC-DAD/ESI-MSn) combined with HPLC-nuclear magnetic resonance (NMR) experiments. Based on this groundwork, a coupling strategy for the comprehensive metabolic profiling of active triterpenoid metabolites from enriched fractions of frankincense was developed. The proposed strategy may serve as a method for the holistic screening of bioactive metabolites in complex TCM samples.
Keywords :
Triterpenoid metabolites , mass spectrometry , Metabolic Profiling , Nuclear magnetic resonance , Frankincense resins
