Determination of hippuric acid in human urine by ion chromatography with conductivity detection

A simple, rapid, precise and eco-friendly ion chromatography (IC) method for the determination of hippuric acid (HA) in human urine was proposed in this paper. The separation was carried out an anion exchange column with 2.0 mmol L−1 Na2CO3 + 2.0 mmol L−1 NaHCO3 as mobile phase at the flow-rate 0.7 mL min−1. A suppressed conductivity detector was used and the detection limit was 1.0 μg L−1(S/N = 3) for hippuric acid. The analysis time for one run was 30 min under the optimized IC condition. The recovery of hippuric acid was 93.2–98.0% while the relative standard deviation (RSD) was 1.4–2.3% by seven measurements.