Title of article
Microdetermination of hyaluronan in human plasma by high-performance liquid chromatography with a graphitized carbon column and postcolumn fluorometric detection
Author/Authors
Toyoda، نويسنده , , Hidenao and Muraki، نويسنده , , Fumie and Imanari، نويسنده , , Toshio and Kinoshita-Toyoda، نويسنده , , Akiko، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2011
Pages
5
From page
950
To page
954
Abstract
A chemical method for the determination of hyaluronan (hyaluronic acid, HA) has been developed and applied to the human blood plasma. Human blood plasma HA was converted to the ΔDi-HA by digestion with hyaluronidase SD and determined by a sensitive and selective high-performance liquid chromatography (HPLC). The HPLC includes the separation and detection of ΔDi-HA using a graphitized carbon column and fluorometric reaction with 2-cyanoacetamide in an alkaline eluent. The calibration graph for ΔDi-HA was linear over the range 0.2 ng–1 μg. It was revealed that the concentration of HA in normal human blood plasma is very low levels (about 24 ng/ml) in comparison to low-sulfated chondroitin 4-sulfate (about 13 μg/ml).
Keywords
Human blood plasma , Graphitized carbon column , Hyaluronan , Fluorometric detection , HPLC
Journal title
Journal of Chromatography B
Serial Year
2011
Journal title
Journal of Chromatography B
Record number
1473028
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