Abstract :
The introduction of a polymeric C30 liquid chromatographic column by Sander et al. [Anal. Chem., 66 (1994) 1667] designed for the separation of carotenoid isomers, has led to the development of improved analytical methods for these compounds. Subsequent commercial availability of polymerically bonded C30 columns has facilitated these advances, and applications to a wide variety of separation problems with biological samples have been described. This report provides a comprehensive review of applications of polymeric C30 columns, utilized in the determination of carotenoids, retinoids, and other nutrients and related compounds in complex, natural-matrix samples.