Soil fulvic acid properties as a means to assess the use of pig slurry amendment

Soil amendment with animal manures is a common practice for either increasing soil organic matter (SOM) and nutrient content or disposing of wastes from intensive animal industries. However, the application of organic amendments that are not sufficiently mature and stable may adversely affect soil properties, especially the content and quality of SOM pools. In this work, the effect of the consecutive annual additions of pig slurry (PS) at rates of 0 (control), 90 and 150 m3 ha−1 per year over a 4-year period on the soil fulvic acid (FA) fraction of SOM was investigated in a field plot experiment conducted under semiarid conditions on a Calcic Luvisol in Toledo province, Spain. The FAs isolated from PS and control and amended soils were characterized for chemical, compositional, structural and functional properties by use of elemental and functional group analysis, and ultraviolet/visible, Fourier transform infrared (FT IR), fluorescence and electron spin resonance (ESR) spectroscopies. PS-FA was characterized by a prevalent aliphatic character, large contents of acidic functional groups, S- and N-containing groups and polysaccharide components, extended molecular heterogeneity, small organic free radical (OFR) contents and small degrees of aromatic ring polycondensation, polymerization and humification. With respect to the control soil FA, the PS-amended soil FAs were characterized by a smaller extraction yield, O and OFR contents and ratios of absorbances at 465 and 665 nm, and larger C, N, S, COOH and phenolic OH contents, C/N ratios and aliphaticity. Statistical analysis of experimental data showed that, with some exceptions, these effects generally increased with increasing cumulative amount of PS applied to soil over time. In conclusion, cumulative PS application to soil over time modifies the content and properties of the FA fraction of SOM. Thus, this material should not be considered as a mature organic amendment and should be treated appropriately before it is applied to soil, so as to increase the degree of humification and enhance its potential as a soil organic fertilizer.