Title of article
Determination of neurotoxin 3-N-oxalyl-2,3-diaminopropionic acid and non-protein amino acids in Lathyrus sativus by precolumn derivatization with 1-fluoro-2,4-dinitrobenzene
Author/Authors
Wang، نويسنده , , Fei and Chen، نويسنده , , Xiong and Chen، نويسنده , , Qian and Qin، نويسنده , , Xinchen and Li، نويسنده , , Zhixiao، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2000
Pages
6
From page
113
To page
118
Abstract
A rapid and simple method is presented for determining neuro-excitatory nonprotein amino acid 3-N-oxalyl-2,3-diaminopropionic acid (β-ODAP) and non-protein amino acids in Lathyrus sativus. Seed and foliage extracts of Lathyrus sativus were treated with 1-fluoro-2,4-dinitrobenzene (FDNB) and a reversed-phase high-performance liquid chromatography method (RP HPLC) for the separation of the derivatives in the pmol range is reported. The RP HPLC method and a colorimetric method were compared for measuring ODAP.
Keywords
Non-protein amino acids , 3-N-Oxalyl-2 , 1-Fluoro-2 , 4-dinitrobenzene , 3-diaminopropionic acid
Journal title
Journal of Chromatography A
Serial Year
2000
Journal title
Journal of Chromatography A
Record number
1501700
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