Title of article :
Determination of the enantiomers of 3-tert.-butylamino-1,2-propanediol by high-performance liquid chromatography coupled to evaporative light scattering detection
Author/Authors :
Toussaint، نويسنده , , B and Duchateau، نويسنده , , A.L.L and van der Wal، نويسنده , , Sj and Albert، نويسنده , , A and Hubert، نويسنده , , Ph. and Crommen، نويسنده , , J، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2000
Pages :
11
From page :
239
To page :
249
Abstract :
A method for the separation and quantitation of the enantiomers of 3-tert.-butylamino-1,2-propanediol by high-performance liquid chromatography and evaporative light scattering detection has been developed. Separation of the enantiomers was performed in normal-phase liquid chromatography on a Chiralpak AS chiral stationary phase. The influence of the gas nature, gas pressure and temperature of the drift tube of the evaporative light scattering detector on the detection sensitivity was investigated. The method was validated in terms of linearity, limit of quantitation, accuracy and precision. The enantiomeric excess of (S)-3-tert.-butylamino-1,2-propanediol, used for the industrial synthesis of (S)-timolol, was measured from 0 to 94%.
Keywords :
3-tert.-Butylamino-1 , 2-Propanediol
Journal title :
Journal of Chromatography A
Serial Year :
2000
Journal title :
Journal of Chromatography A
Record number :
1502452
Link To Document :
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