Title of article
New method for high-performance liquid chromatographic separation and fluorescence detection of ginsenosides
Author/Authors
Shangguan، نويسنده , , Dihua and Han، نويسنده , , Huiwan and Zhao، نويسنده , , Rui and Zhao، نويسنده , , Yingxin and Xiong، نويسنده , , Shaoxiang and Liu، نويسنده , , Guoquan، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2001
Pages
6
From page
367
To page
372
Abstract
A novel pre-column derivatization method for the quantitative determination of ginsenosides by HPLC with fluorescence detection was established. The double bond at the C24–C25 position of ginsenoside was converted into an aldehyde group by means of ozonolysis. Then the aldehyde group reacts with FMOC-hydrazine forming the ginsenoside FMOC-hydrazone. The derivatized products were separated by RP-HPLC with gradient elution. The detection limits of ginsenosides Rg1 and Rb1 were 2.0 ng (about 2.5 pmol) and 1.0 ng (about 0.9 pmol), respectively. This method can be used for all ginsenosides having the C24–C25 double bond.
Keywords
Ginsenosides
Journal title
Journal of Chromatography A
Serial Year
2001
Journal title
Journal of Chromatography A
Record number
1506046
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