New configuration in capillary isotachophoresis–capillary zone electrophoresis coupling

The paper surveys possible configurations of a coupling capillary column operating in various electromigration modes. Special attention is given to capillary isotachophoresis–capillary zone electrophoresis (cITP–CZE) coupling and its description from the theoretical point of view. Computer simulations of separation are presented and compared with experiments. Further, we propose a new configuration of electrolyte systems in cITP–CZE coupling, which offers a possibility to perform complex analyses of micro- and macro-constituents in one run. The electrolyte system is verified by practical experiments for both anionic and cationic modes of analysis. The advantages and disadvantages of the new combination are discussed.