Non-aqueous capillary electrophoretic separation of enantiomeric amines with (−)-2,3:4,6-di-O-isopropylidene-2-keto-l-gulonic acid as chiral counter ion

(−)-2,3:4,6-Di-O-isopropylidene-2-keto-l-gulonic acid [(−)-DIKGA] has been introduced as a chiral counter ion in non-aqueous capillary electrophoresis. High enantioresolutions (RS≥3) were obtained for amines, e.g., pronethalol, labetalol and bambuterol. Methanol containing NaOH and (−)-DIKGA was used as the background electrolyte. The counter ion concentration and the nature of the injection medium were found to affect the chiral separation. Covalent coating of the fused-silica capillary reduced the electro-osmotic flow resulting in improved enantioresolutions.