Ultrasensitive chemiluminescence detection of sub-fM level Co(II) in capillary electrophoresis

A method of on-line ultrasensitive chemiluminescence detection with capillary electrophoresis for Co(II) is reported. Using our newly developed capillary electrophoresis with chemiluminescence detection system and novel mixing mode of the reagents, the effects of field-amplified injection on detection limits of metal ions were studied in detail. The sub-fM level (1.3·10−16 M, 1.6×10−24 mol, 1 molecule) detection of cobalt ions in ultradilute solution was performed. The catalytic behavior of the chemiluminescence reaction of luminol and hydrogen peroxide by cobalt ions and the reaction conditions, such as the concentration of luminol, H2O2, and pH of chemiluminescence reagent were investigated. The separation of fM level Co(II) and trace amounts of Ni(II) was performed successfully.