Author/Authors :
Yamamoto، نويسنده , , Atsushi and Akiba، نويسنده , , Nami and Kodama، نويسنده , , Shuji and Matsunaga، نويسنده , , Akinobu and Kato، نويسنده , , Kayoko and Nakazawa، نويسنده , , Hiroyuki، نويسنده ,
Abstract :
A multi-beam circular dichroism (CD) detector which is easily constructed by inserting inexpensive optics into a conventional photo-diode array detector has an advantage of simultaneous detection of the absorbance and CD. The enantiomeric purity determination of malic acid in beverages was performed by this detection system. Malic acid when complexed with Cu(II) was found to have an absorbance maximum at around 750 nm. The l-malic acid–Cu(II) complex showed a positive Cotton effect in its absorbance band and its anisotropy factor (Δε/ε) was relatively large at about 1/170. This complex was retained on a reversed-phase column with the addition of racemic 2-hydroxy-3-methylbutyric acid to the mobile phase as the ligand. A plot of the relative peak areas between the CD and the absorption (Δabs/abs) versus optical purity showed good linearity with a correlation coefficient of 0.999, and the precision expressed as the relative standard deviation of the errors from the regression line was ±2.7% (2σ). The accuracy of the proposed method was assessed by capillary electrophoresis. Eight commercially available juice products were analyzed using this method. Five of them were thought to be adulterated with synthetic malic acid.
