High-performance liquid chromatographic stationary phases based on poly(methyloctylsiloxane) immobilized on silica: II. Chromatographic evaluation

This work describes the chromatographic characterization of stationary phases prepared by deposition of poly(methyloctylsiloxane) (PMOS) on silica followed by immobilization using one of several different processes: thermal treatments (120 or 220 °C for 4 h), microwave irradiation (495 W for 15 min), γ radiation (dose of 80 kGy) or self-immobilization. This evaluation was based on the chromatographic parameters of several test solutes. The stationary phases immobilized at 220 °C and which underwent self-immobilization were not appropriate for chromatographic use but the other immobilized phases presented chromatographic performances similar in most respects to a commercial phase (Rainin C8) while the peak characteristics of the basic probe were significantly better with these phases.