Author/Authors :
Theodoridis، نويسنده , , G and Haasnoot، نويسنده , , W and Cazemier، نويسنده , , G and Schilt، نويسنده , , R and Jaziri، نويسنده , , M and Diallo، نويسنده , , B and Papadoyannis، نويسنده , , I.N and de Jong، نويسنده , , G.J، نويسنده ,
Abstract :
The application of immunoaffinity chromatography for the purification of Taxus plant and cell extracts prior to the HPLC analysis is described. Polyclonal antibodies raised against 10-deacetylbaccatin III (10-DAB III), paclitaxel’s main precursor in plant, were characterised by enzymed-linked immunosorbent assay. Immunoglobulins from selected antisera were immobilised on CNBr-activated Sepharose 4B. The immunoaffinity column was used for the purification of plant and plant cell culture extracts prior to their analysis by HPLC. Immunoaffinity chromatography enabled the selective concentration of taxoids and enhanced sample clean-up.
Keywords :
taxol , Deacetylbaccatin III , Taxanes , Paclitaxel
