Author/Authors :
Song، نويسنده , , Shuang and Sun، نويسنده , , Lu and Yuan، نويسنده , , Lei-lei Sun، نويسنده , , Tiemin and Zhao، نويسنده , , Yun and Zuo، نويسنده , , Wenjian and Cong، نويسنده , , Yue and Li، نويسنده , , Xian and Wang، نويسنده , , Jinhui، نويسنده ,
Abstract :
Using nonchiral high-performance liquid chromatography (HPLC) coupled with a circular dichroism (CD) detector, we developed a new method to determine the enantiomeric excess (e.e.) of glucose derivatized with chromophores (p-bromobenzoate). This method is suitable for microscale analysis of glycoside and saccharide. As an application of this method, the enantiomeric excess of the component glucose of a glucan was assayed.
Keywords :
Enantiomeric excess , Glucose , absolute configuration , detector , circular dichroism , HPLC-CD
