Author/Authors :
Jensen، نويسنده , , Pia H. and Juhler، نويسنده , , René K. and Nielsen، نويسنده , , Nikoline J. and Hansen، نويسنده , , Thomas H. and Strobel، نويسنده , , Bjarne W. and Jacobsen، نويسنده , , Ole S. and Nielsen، نويسنده , , John and Hansen، نويسنده , , Hans Christian B. Hansen، نويسنده ,
Abstract :
Potato glycoalkaloids are produced in high amounts in potato fields during the growth season and losses to soil potentially impact shallow groundwater and via tiles to fresh water ecosystems. A quantitative liquid chromatography–electrospray ionization time-of-flight mass spectrometry (LC–ESI-TOF-MS) method for determination and quantification of potato glycoalkaloids and their metabolites in aqueous soil extracts was developed. The LC–ESI-TOF-MS method had linearities up to 2000 μg/L for α-solanine and α-chaconine and up to 760 μg/L for solanidine. No matrix effect was observed, and the detection limits found were in the range 2.2–4.7 μg/L. The method enabled quantification of the potato glycoalkaloids in environmental samples.
Keywords :
Natural Toxins , LC–MS , soil extract , ?-Solanine , ?-Chaconine , Solanidine
