Author/Authors :
Hoang، نويسنده , , Ngoc-Ly and Landolfi، نويسنده , , Antonin and Kravchuk، نويسنده , , Anastasiya and Girard، نويسنده , , Etienne and Peate، نويسنده , , Jonathan and Hernandez، نويسنده , , Javier M. and Gaborieau، نويسنده , , Marianne and Kravchuk، نويسنده , , Olena and Gilbert، نويسنده , , Robert G. and Guillaneuf، نويسنده , , Yohann and Castignolles، نويسنده , , Patrice، نويسنده ,
Abstract :
The structure of starch molecules is relevant to nutrition and industrial applications. Size-exclusion chromatography (SEC, also known as GPC) of native starch generally suffers non-satisfactory repeatability and reproducibility of the dissolution and separation. This work combines two polar organic solvents: dimethylsulfoxide for complete dissolution and dimethylacetamide to limit shear degradation. The separation is as repeatable as that of polystyrene standards performing dissolution and separation at 80 °C. Successful covalent-labeling on the glucose unit is claimed to be published here for the first time in non-degradative conditions and allows the use of UV detector with significantly higher sensitivity than with a refractometer.
Keywords :
UV detection , Starch , Repeatability , Separation , Labeling , Size-exclusion chromatography (SEC)
