Application of acetate derivatives for gas chromatography–mass spectrometry: Novel approaches on carbohydrates, lipids and amino acids analysis

The structure of glycoconjugates has been determined by several chromatographic methods, however gas chromatography–mass spectrometry (GC–MS) has been widely used to identify and quantify the volatile trimethylsilyl and fluoroacyl derivatives. Adapting the reduction/acetylation strategies, we had performed the derivatization of all monosaccharide class, as well as amino acids and OH-fatty acids as from different glycoconjugates. Uronic acids gave characteristic ions at m/z 143, 156 and 173, and 19 amino acids derivatives, gave molecular ions [M]+ and daughter ions of [M−59]+ and [M−43]+ on electron impact (EI)–MS, which provide their rapid identification.