New gas chromatographic method for the enantioseparation of β-amino acids by a rapid double derivatization technique

A novel gas chromatographic method was developed for the enantioseparation of valuable acyclic and carbocyclic cis- and trans-β-amino acids, including cispentacin and a number of its analogues and homologues. Excellent (in most cases baseline) separation was achieved for the racemates of these β-amino acids on CP-Chirasil-Dex CB or CP-Chirasil L-Val columns after a simple and rapid double derivatization (esterification followed by N-acylation). The elution sequences were determined in all cases.