Title of article :
Hollow fiber liquid-phase microextraction coupled with gas chromatography-flame ionization detection for the profiling of fatty acids in vegetable oils
Author/Authors :
Siang، نويسنده , , Gan Hui and Makahleh، نويسنده , , Ahmad and Saad، نويسنده , , Bahruddin and Lim، نويسنده , , Boey Peng Lim، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2010
Pages :
6
From page :
8073
To page :
8078
Abstract :
The development of a two phase hollow fiber liquid-phase microextraction technique, followed by gas-chromatography-flame ionization detection (GC-FID) for the profiling of the fatty acids (FAs) (lauric, myristic, palmitic, stearic, palmitoleic, oleic, linoleic, linolenic and arachidic) in vegetable oils is described. Heptadecanoic acid methyl ester was used as the internal standard. The FAs were transesterified to their corresponding methyl esters prior to the extraction. Extraction parameters such as type of extracting solvent, temperature, extraction time, stirring speed and salt addition were studied and optimized. Recommended conditions were extraction solvent, n-tridecane; extraction time, 35 min; extraction temperature, ambient; without addition of salt. Enrichment factors varying from 37 to 115 were achieved. Calibration curves for the nine FAs were well correlated (r2 > 0.994) within the range of 10–5000 μg L−1. The limit of detection (signal:noise, 3) was 4.73–13.21 ng L−1. The method was successfully applied to the profiling of the FAs in palm oils (crude, olein, kernel, and carotino cooking oil) and other vegetable oils (soybean, olive, coconut, rice bran and pumpkin). The encouraging enrichments achieved offer an interesting option for the profiling of the minor and major FAs in palm and other vegetable oils.
Keywords :
Liquid phase microextraction , GC-FID , fatty acids , palm oil , Vegetable oils
Journal title :
Journal of Chromatography A
Serial Year :
2010
Journal title :
Journal of Chromatography A
Record number :
1513638
Link To Document :
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