Title of article :
Simultaneous determination of flonicamid and its metabolites in vegetables using QuEChERS and reverse-phase liquid chromatography–tandem mass spectrometry
Author/Authors :
Xu، نويسنده , , Yong and Shou، نويسنده , , Lin-Fei and Wu، نويسنده , , Yin-Liang، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
Pages :
4
From page :
6663
To page :
6666
Abstract :
This paper presents a rapid analytical method for the simultaneous determination of flonicamid and its metabolites N-(4-trifluoromethylnicotinoyl) glycine (TFNG), 4-trifluoromethylnicotinic acid (TFNA), and 4-trifluoromethylnicotinamide (TFNA-AM) in vegetables using QuEChERS by liquid chromatography–tandem mass spectrometry (LC–MS/MS). Samples were extracted with acetonitrile. The extract was purified through QuEChERS method with primary secondary amine (PSA) and graphite carbon black (GCB). Then the extract was diluted with 0.1% formic acid in water, and analyzed by LC–MS/MS on a Waters Acquity BEH C18 column with methanol/0.1% formic acid in water as mobile phase with gradient elution. The linearity of the analytical response across the studied range of concentrations (0.20–500 μg/L) was excellent, obtaining correlation coefficients higher than 0.998. No significant matrix effects were observed. Recovery studies were carried out on spiked spinach and cucumber blank samples, at four concentration levels (0.01, 0.05, 0.50 and 2.0 mg/kg) performing six replicates at each level. Mean recoveries of 81.3–94.8% with CVs of 2.4–7.0% were obtained. The method demonstrated to be suitable for the simultaneous determination of flonicamid and its metabolites in vegetables.
Keywords :
Flonicamid , LC–MS/MS , QuEChERS , Insecticide , Vegetables
Journal title :
Journal of Chromatography A
Serial Year :
2011
Journal title :
Journal of Chromatography A
Record number :
1514476
Link To Document :
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