Title of article :
Antibody capture by mixed-mode chromatography: A comprehensive study from determination of optimal purification conditions to identification of contaminating host cell proteins
Author/Authors :
Pezzini، نويسنده , , Jérôme and Joucla، نويسنده , , Gilles and Gantier، نويسنده , , René and Toueille، نويسنده , , Magali and Lomenech، نويسنده , , Anne-Marie and Le Sénéchal، نويسنده , , Caroline and Garbay، نويسنده , , Bertrand and Santarelli، نويسنده , , Xavier and Cabanne، نويسنده , , Charlotte، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
Pages :
12
From page :
8197
To page :
8208
Abstract :
We evaluated mixed mode chromatography for the capture of recombinant antibodies from CHO cell culture supernatants. We studied PPA HyperCel, HEA HyperCel, MEP HyperCel and Capto adhere resins, which all contain hydrophobic and cationic groups. A microplate approach combined with DoE modeling allowed the exploration of the complex behaviors of these mixed mode resins. Optimal conditions for antibody purification and host cell proteins (HCPs) elimination were determined and then directly up-scaled to laboratory columns. Then we used mass spectrometry to identify the major HCPs potentially coeluted with the antibody. Differences between the four resins in terms of amount, complexity and identity of the HCPs present in the elution fractions were investigated.
Keywords :
Design of Experiment , Mixed-mode chromatography , mass spectrometry , antibody , Host cell proteins , Screening
Journal title :
Journal of Chromatography A
Serial Year :
2011
Journal title :
Journal of Chromatography A
Record number :
1514655
Link To Document :
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