Author/Authors :
Hu، نويسنده , , Ting and Peng، نويسنده , , Tao and Li، نويسنده , , Xiaojuan and Chen، نويسنده , , Dong-Dong and Dai، نويسنده , , Han-Hui and Deng، نويسنده , , Xiao-Jun and Yue، نويسنده , , Zhen-Feng and Wang، نويسنده , , Guo-Min and Shen، نويسنده , , Jianzhong and Xia، نويسنده , , Xi and Ding، نويسنده , , Shuang-Yang and Zhou، نويسنده , , Yue-Ning and Zhu، نويسنده , , Ai-Ling and Jiang، نويسنده , , Hai-Yang، نويسنده ,
Abstract :
An ultra-high-performance liquid chromatography with tandem mass spectrometric detection (UHPLC–MS/MS) method was established for the simultaneous determination of residues of thirty non-steroidal anti-inflammatory drugs (NSAIDs) in swine muscle. The samples were extracted with acetonitrile and phosphoric acid. The extracts were defatted with n-hexane, and then purified by HLB solid-phase extraction cartridge. Analysis was carried out on UHPLC–ESI-MS/MS working with multiple reaction monitoring mode with polarity switching. Limits of detection were between 0.4 μg/kg and 2.0 μg/kg, and limits of quantification were between 1.0 μg/kg and 5.0 μg/kg. The recoveries of NSAIDs were between 61.7% and 125.7% at spiked levels of 1.0–500 μg/kg. The repeatability was less than 8% and the within-laboratory reproducibility was not more than 12.3%. The method was reliable, convenient and sensitive.
Keywords :
Swine muscle , Non-steroidal anti-inflammatory Drugs , multi-residue analysis , Tandem mass spectrometry , Ultra-high-performance liquid chromatography
