Title of article :
Enantioseparation of racemic trans-δ-viniferin using high speed counter-current chromatography based on induced circular dichroism technology
Author/Authors :
Han، نويسنده , , Chao and Xu، نويسنده , , Jinfang and Wang، نويسنده , , Xiaobing and Xu، نويسنده , , Xiaoming and Luo، نويسنده , , Jianguang and Kong، نويسنده , , Lingyi، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2014
Abstract :
A preparative chiral high speed counter-current chromatography (HSCCC) method based on induced circular dichroism (ICD) spectrum was developed to separate trans-δ-viniferin (TVN) enantiomers successfully. The important parameters for the chiral HSCCC separation process, including the type of chiral selector (CS), the concentration of chiral selector and the equilibrium temperature, were optimized using induced circular dichroism spectrum. The final separation procedure was established with a biphasic solvent system composed of n-hexane–ethyl acetate–25 mmol L−1 hydroxypropyl-β-cyclodextrin aqueous solution (5:5:10, v/v/v) in the head-to-tail elution mode at 5 °C. Under optimum chiral HSCCC separation conditions, 8.2 mg of (7S, 8S)-TVN (1) and 9.4 mg of (7R, 8R)-TVN (2) were successfully separated from 20 mg TVN enantiomers with the purity of 99.51% and 99.36%, respectively.
Keywords :
Chiral separation , High speed counter-current chromatography , Trans-?-viniferin , Induced circular dichroism spectrum , Hydroxypropyl-?-cyclodextrin
Journal title :
Journal of Chromatography A
Journal title :
Journal of Chromatography A