Single column approach for the liquid chromatographic separation of polar and non-polar glucosinolates from broccoli sprouts and seeds

Ion-pair and hydrophilic interaction chromatographies are considered to be complementary methods of choice for analyzing intact glucosinolates from broccoli. Ion-pair chromatography resolves non-polar glucosinolates, such as those containing indole moieties, while hydrophilic interaction chromatography is superior for separating polar glucosinolates, such as glucoraphanin and glucoiberin. Reversed-phase separations using hydrophilic endcapped C18-bonded silica and a 50 mM ammonium acetate–methanol gradient mobile phase resolve both polar and non-polar glucosinolates negating the need for switching columns.