Author/Authors :
Chetiyanukornkul، نويسنده , , Thaneeya and Toriba، نويسنده , , Akira and Kizu، نويسنده , , Ryoichi and Makino، نويسنده , , Tsunehisa and Nakazawa، نويسنده , , Hiroyuki and Hayakawa، نويسنده , , Kazuichi، نويسنده ,
Abstract :
A high-performance liquid chromatographic method has been developed for the quantification of 1-hydroxypyrene (1-OHP) in human urine using deuterated 1-hydroxypyrene ([2H9]1-OHP) as an internal standard with fluorescence detection. [2H9]1-OHP was prepared enzymatically from deuterated pyrene ([2H10]Pyr) with cytochrome P450 1A1. It eluted immediately prior to non-deuterated 1-OHP on alkylamide-type reversed-phase columns and had nearly the same fluorescence characteristics as non-deuterated 1-OHP. The detection limit was 0.1 μg/L and the calibration range was from 1 to100 nmol/L. Urine sample treatment involved enzymatic hydrolysis followed by solid-phase extraction using Sep-Pak C18 cartridges. The proposed method was used to determine urinary 1-OHP in smokers and non-smokers.
