Analysis of wine phenolics by high-performance liquid chromatography using a monolithic type column

A HPLC method involving direct injection was developed to determine phenolic compounds in wine. The separation was carried out on an RP C18 monolithic column using a binary gradient elution and diode array detection. In this way 17 monomeric compounds of different phenolic groups (hydroxybenzoic acids, hydroxycinnamic acids, hydroxycinnamyltartaric acids, flavanol, flavonol and stilbenes) could be separated and quantified in a single run and in a very short time.