Author/Authors :
واني، عبدالرئوف نويسنده Department of Animal Reproduction, Faculty of Veterinary Sciences and Animal Husbandry Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India Wani, A. R , خان، محمد ظفراله نويسنده Department of Animal Reproduction, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India Khan, M. Z , صوفي، خورشيد احمد نويسنده Department of Animal Reproduction, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India Sofi, K. A , مالك، اسلوب احمد نويسنده Department of Animal Reproduction, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India Malik, A. A , لون، فاروض احمد نويسنده Department of Animal Reproduction, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India Lone, F. A , بات، فياض احمد نويسنده Department of Animal Reproduction, Faculty of Veterinary Sciences and Animal Husbandry Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India Bhat, F. A
Abstract :
A study was conducted to investigate the effect of follicular size (small, <2 mm diameter and large, ≥2 mm) on in vitro maturation, fertilization and culture of ovine oocytes. The ovaries (n=80) were collected from abattoirs in Srinagar city, India and transported in normal saline solution containing 50 μg gentamicin sulphate per ml in a thermos flask. The oocytes were collected by puncture technique and good and fair quality oocytes obtained were subjected to maturation in TCM-199 supplemented with epidermal growth factor (EGF) for 24 h under 5% CO2 and humidified atmosphere at 38.5 C. The matured oocytes were then subsequently subjected to fertilization and culturing was done for six days. The results revealed that the maturation rate of large follicle oocytes (85.20%) was higher than small follicle oocytes (52.44%, P=0.002). In vitro fertilization rates for small and large follicle oocytes were not significantly different (79.54 and 76.65%, respectively). However, cleavage and morula rates were higher in large follicle oocytes (58.69 and 34.13%) than small follicle oocytes (38.63 and 19.32%, respectively, P<0.05). In conclusion, there is higher developmental competence of oocytes from the large follicles than small follicles and the pre-maturation of oocyte occurring during follicular growth is essential for subsequent embryonic development in sheep.
