Author/Authors :
Massip، نويسنده , , C and Riollet، نويسنده , , P and Quemener، نويسنده , , E and Bayle، نويسنده , , C and Salvayre، نويسنده , , R and Couderc، نويسنده , , F and Caussé، نويسنده , , E، نويسنده ,
Abstract :
In this work, we will present some attempts to analyze tyrosine and nitrotyrosine using capillary electrophoresis and either UV–Visible detection or laser-induced fluorescence (LIF) detection. An argon ion (488 nm) laser is used for fluorescein isothiocyanate (FITC) and 7-fluoro-4-nitro-2,1,3-benzoxadiazole (NBD-F). A near infrared (780 nm) laser is used for NIR 780 derivatives. The UV–Visible limit of detection is 2.5 μM whereas it is in the range of 30 nM for LIF detection.
