Study of solute partitioning into cationic vesicles of dihexadecyldimethylammonium bromide using electrokinetic chromatography

A cationic vesicular pseudo-stationary phase was used in electrokinetic chromatography. The stable cationic lipid bilayer, composed of a double chain cationic surfactant, dihexadecyldimethylammonium bromide was prepared in deionized water, with no buffer additive. The chromatographic characteristics of this novel pseudo-phase are presented. A linear solvation energy relationship was successfully used to characterize the interaction of neutral solutes with the cationic vesicles. This study was complemented by the determination of changes in the free energy for transfer of various groups from the aqueous phase into the bilayer.